Utrecht University
Weingarth Lab
Biological and Biomedical Solid-State NMR
We use state-of-the-art solid-state NMR methods, often in combination with computational techniques, to study medically important biomolecules and biomaterials at the atomic level and at native (cellular) conditions. Our main research interests are:
Selected Recent Publications
1. Jekhmane, S., Medeiros-Silva, J., Li, J., Kümmerer, F., Müller-Hermes, C., Baldus, M., Roux, B., Weingarth., M., (2019) Nature Comm., 10, 12, Shifts in the selectivity filter dynamics cause modal gating in K+ channels See also Press release
2. Medeiros-Silva, J., Jekhmane, S., Paioni, A., Gawarecka, K., Baldus, M., Swiezewska, E., Breukink, E., Weingarth, M., (2018) Nature Comm., 9, 3963, High-resolution NMR studies of antibiotics in cell membranes See here for the Press release & coverage & coverage
3. Visscher, K.M., Medeiros-Silva, J. Mance, D., Rodrigues, J.P.G.L.M., Daniëls, M., Bonvin, A.M.J.J., Baldus, M., Weingarth, M., (2017) Angew. Chem., 56, 13222, Supramolecular organization and functional implications of K+ channel clusters in membranes Selected as Frontispiece
4. Rad Malekshahi, M., Visscher. K.M., Rodrigues J.P.G.L.M., de Vries, R., Hennink, W.E., Baldus, M., Bonvin A.M.J.J., Mastrobattista E., Weingarth. M. (2015) J. Am. Chem. Soc., 137, 7775, The supramolecular organization of a peptide based nanocarrier at high molecular detail
5. Sinnige, T., Daniels, M., Baldus, M.,Weingarth, M. (2014) J. Am. Chem. Soc., 136, 4452, Proton clouds to measure non-exchangeable sidechain protons in solid-state NMR Article featured the Cover of JACS
Shehrazade Jekhmane & João Medeiros-Silva et al., Nature Communications (2019) 10, 12, p. 1-11
Shifts in the selectivity filter dynamics cause modal gating in K+ channels
This work provides a
long-needed quantitative description of the selectivity filter dynamics in a
native environment, which is of fundamental importance to understand ion channel function
João Medeiros-Silva et al.,
Nature Communications (2018)
9, 3963, p. 1-10
High-resolution NMR studies of antibiotics in cellular membranes
A powerful NMR approach (integrating proton-detection and 800 MHz DNP) to study promising membrane-active antimicrobial peptides at high-resolution and directly in cellular conditions.
Angewandte Chem. (2017)
56, 13222-13227
A DNP-ssNMR approach to study membrane protein - membrane protein interactions in relation to function in a FRET-like manner
