Utrecht University

Biomedical Solid-State NMR

Utrecht University

Weingarth Lab    

Biomedical Solid-State NMR

We use state-of-the-art solid-state NMR methods, often in combination with computational techniques, to study medically important biomolecules and biomaterials at the atomic level and at native (cellular) conditions. Our main research interests are:

  • Antibiotics
  • Bioactive peptides (tissue engineering & drug delivery)
  • Membrane proteins (especially ion channels)

Selected Recent Publications 

1. Jekhmane, S., Medeiros-Silva, J., Li, J., Kümmerer, F., Müller-Hermes, C., Baldus, M., Roux, B., Weingarth., M., (2019) Nature Comm., 10, 12, Shifts in the selectivity filter dynamics cause modal gating in K+ channels See also Press release

2. Medeiros-Silva, J., Jekhmane, S.,  Paioni, A., Gawarecka, K., Baldus, M., Swiezewska, E., Breukink, E., Weingarth, M., (2018Nature Comm., 9, 3963, High-resolution NMR studies of antibiotics in cell membranes  See here for the Press release & coverage & coverage

3. Visscher, K.M.,  Medeiros-Silva, J. Mance, D., Rodrigues, J.P.G.L.M., Daniëls, M., Bonvin, A.M.J.J., Baldus, M., Weingarth, M., (2017) Angew. Chem., 56, 13222, Supramolecular organization and functional implications of K+ channel clusters in membranes  Selected as Frontispiece 

4. Rad Malekshahi, M., Visscher. K.M., Rodrigues J.P.G.L.M., de Vries, R., Hennink, W.E.,  Baldus, M., Bonvin A.M.J.J., Mastrobattista E., Weingarth. M. (2015) J. Am. Chem. Soc., 137, 7775, The supramolecular organization of a peptide based nanocarrier at high molecular detail 

5. Sinnige, T., Daniels, M., Baldus, M.,Weingarth, M. (2014) J. Am. Chem. Soc., 136, 4452, Proton clouds to measure non-exchangeable sidechain protons in solid-state NMR  Article featured the Cover of JACS

Shehrazade Jekhmane & João Medeiros-Silva  et al.,  Nature Communications (2019) 10, 12, p. 1-11 

Shifts in the selectivity filter dynamics cause modal gating in K+ channels

This work provides a long-needed quantitative description of the selectivity filter dynamics in a native environment, which is of fundamental importance to understand ion channel function

Press release 

João Medeiros-Silva  et al., 

Nature Communications (2018)

9, 3963, p. 1-10 

High-resolution NMR studies of antibiotics in cellular membranes

A powerful NMR approach (integrating proton-detection and 800 MHz DNP) to study promising membrane-active antimicrobial peptides at high-resolution and directly in cellular conditions.

Press release (EnglishDutch)

Koen Visscher et al. 

Angewandte Chem. (2017) 

56, 13222-13227

A DNP-ssNMR approach to study membrane protein - membrane protein interactions in relation to function in a FRET-like manner