Utrecht University

Biological Solid-State NMR

Utrecht University

K+ channels cluster in reference to their gating cycle

Using a combination of dynamic nuclear polarisation (DNP) enhanced solid-state NMR measurements in bacterial membranes and large-scale computer simulations, we could recently show that K+ channels aggregate and dissolve  in reference to their gating cycle, uncovering a hitherto unknown mechanism of functional coupling between K+ channels.

Check out the paper in Angewandte Chemie: Visscher et al. 2017

K+ channels: unexpected  conformational dynamics

The selectivity filter of potassium channels is highly conserved throughout bacteria and eukaryotes. Using modern 1H-detected solid-state NMR, we demonstrate that the conductive selectivity  filter exhibits substantially increased slow microsecond motion. Such slow motion is on the same time-scale as thus far unexplained gating modes such as flickering.

Check out the paper in Angewandte Chemie: Medeiros-Silva et al. 2016  

K+ channel - water interaction...

Check out the papers in JACS 2014  and Angewandte Chemie 2015

K+ channel - lipid interaction...

....to come